Frontiers isolation and characterization of a phosphorus. Isolation and assessment of nitrogenfixing and phosphate solubilizing bacteria for use as biofertilizers. Occurrence of phosphate solubilizing bacteria high proportion of psm is concentrated in the rhizosphere, and they are metabolically more active than from other sources. The pgpr xsb375 enhances seed germination, early emergence, plant vigor, root and shoot growth, and. Isolation and evaluation of phosphate solubilizing.
Isolation and identification of elite phosphate solubilizing bacteria from soil under paddy cultivation a. Phosphate solubilizing fungi play a noteworthy role in increasing the bioavailability of soil phosphates for plants. Isolation and characterization of a phosphatesolubilizing. Isolation of phosphate solubilizing bacteria and their ability to solubilize calcium phosphate from the rhizosphere soils tomato, jasmine, onion, and maize, the total of 12 bacterial species of 32 samples were found enriched on pikovskayas agar plates. Pdf isolation and screening of phosphate solubilizing. From isolation of phosphate solubilizing microbes to their. Isolation and identification of potential phosphate solubilizing bacteria from the rhizoplane of oryza sativa l. Collected soil samples were stored in polythene bags aseptically and maintained at the laboratory for further study. Isolation and characterization of phosphate solubilizing bacteria from paddy field of bhilai region. Isolation and characterization of phosphate solublizing. Isolation of phosphate solubilizing bacteria and fungi. One fungal strain was found to be efficient phosphorus solubilizer in pikovskaya medium. Phosphorus is an essential element for plant development and growth making up about 0. The soil samples used for bacterial isolation were collected from rhizospheric soil fractions and rootfree soils from the salinity affected two.
A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. A study on isolation of phosphate solubilizing bacterial. Modi2 1department of biotechnology, kadi sarva vishwavidyalaya,gandhinagar 2life science department, school of sciences, gujarat university,ahmedabad author for correspondence abstract. Isolation and identification of phosphate solubilizing. In this regard, the ability of phosphofungi to dissolve insoluble phosphorus compounds is greater than that of phosphate solubilizing bacteria. The use of phosphate solubilizing pgpr as inoculants is one of the alternative biotechnological solutions in sustainable agriculture to meet the phosphate demands of.
Isolation and characterization of a phosphorussolubilizing. Isolation and characterization of phosphate solubilizing pseudomonas sp. Isolation and characterization of ralstonia pickettiia. Coochbehar, jalpaiguri and alipurduar from different crop rhizosphere. Phosphate solubilizing bacteria psb have the potential to enhance phosphate induced immobilization of metals to remediate contaminated soil. In this study, isolation, screening and characterization of 35 isolates of phosphate solubilizing bacteria from different regions of. A study on isolation of phosphate solubilizing bacterial psb. Twenty phosphate solubilizing bacteria psb were isolated from calcareous rhizosphere soils. Isolation of phosphate solubilizing microorganism psms from soil. Homogenization of soil was carried out by keeping it on shaker for 30 minutes at 120 rpm at 27oc temperature. Eight bacteria and 8 fungi possessed solubilizing ability. Nineteen soil samples were collected from different terai agroecological region of west bengal i. Abstractphosphate solubilizing bacteria psb play a significant role in plant p nutrition by their effect on soil p dynamics and their subsequent ability to make p available to plants via solubilization and mineralization processes.
The soil bacteria belonging to the genera pseudomonas and bacillus and fungi are more common role of biofertilizer in organic agriculture. A considerably higher concentration of phosphate solubilizing bacteria is commonly found in the rhizosphere in comparison with. One method is by promoting nutrient acquisition from soil. Phosphate solubilizing fungi and bacteria are known as effective organisms in this process. This study aimed to isolate a highefficient level of phosphatesolubilizing fungus from rhizosphere soil samples phosphate mines liuyang county, hunan province, china and apply it in solubilization of rps. For isolation of psf from bulk or rhizosphere soil, approximately 10 g bulk soil or root plus adhering soil were transferred into 250 ml erlenmeyer.
Potassium is a vital component of plant nutrition package limiting crop yield and. It is the least mobile element in plant and soil contrary to other macronutrients. In the present study total 28 phosphate solubilising bacteria were isolated from rhizospheric soil of neem, mango and jatropha plant. Pseudomonas cepacia and penicillium purpurogenum showed the highest activity. Isolation of potential phosphate solubilizing bacteria from. Isolation of phosphate solubilizing bacteria psb from rhizosphere soil soil samples were prepared by inoculating 1. It could produce organic acids, protons, and enzymes that transform insoluble phosphates into easily assimilated forms by plants that may ameliorate phosphorus supply to the soil 21. In the present work we have made efforts to isolate the phosphate solubilizing microorganism form soil followed by its biochemical and molecular characterization. Nagalakshmi department of microbiology, kanchi shri krishna college of arts and science, kilambi, kanchipuram631551, tn, india corresponding author abstract introduction phosphorus p is a major growthlimiting. Oct 06, 2014 woo sm, lee mk, hong is, poonguzhali s, sa tm 2010 isolation and characterization of phosphate solubilizing bacteria from chinese cabbage.
In this context, the management of phosphates solubilizing and n 2. Isolation and characterization of phosphatesolubilizing. Isolation and characterization of halotolerant phosphate solubilizing bacteria naturally colonizing the peanut rhizosphere in saltaffected soil author. Isolation of phosphate solubilizing microorganism psms. Isolation of phosphate solubilizing bacterial strain phosphate solubilizing bacterial strain isolated from a jute mill effluent mixing area of ganga river water at bansberia 2258. Isolation and characterization of phosphate solubilizing bacteria from phosphate solid sludge of the moroccan phosphate mines. Several scientists have reported the ability of different bacterial species to solubilize insoluble inorganic phosphate compounds, such as tricalcium phosphate, dicalcium phosphate, hydroxyapatite, and rock phosphate. Soil samples were collected from the maize rhizosphere soils of maize research station and college farm, college of agriculture, pjtsau, rajendranagar, hyderabad. Isolation and characterization of phosphate solubilizing microbes from agricultural soil t. Isolation and characterization of ralstonia pickettii a novel phosphate solubilizing bacterium from pomegranate rhizosphere from western india. Different strains of phosphate solubilizing bacteria psb were isolated from the rhizosphere of different plants of lahore district, pakistan. Occurrence and genetic diversity of phosphate solubilizing bacteria in soils of differing chemical characteristics in kenya author. Isolation and identification of potential phosphate. Biochemical and molecular characterization of potential.
Pdf isolation of phosphate solubilizing microorganism. Forty two soil isolates 31 bacteria and 11 fungi were studied for their ability to solubilize rock phosphate and calcium phosphate in culture medium. A total of 29 composite rhizosphere soil samples were used for the isolation of. Psb were identified based on 16s rrna gene sequencing as pseudomonas synxantha, burkholderia gladioli, enterobacter hormaechei and serratia marcescens. Isolation and characterization of mineral potassium. Isolation of phosphate solubilizing bacteria and fungi from. All the samples were analyzed for the presence of psb in laboratory by adopting standard procedure.
Phosphate solubilizing bacteria psb play an important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments. Panjabrao deshmukh krishi vidyapeeth, akola, maharashtra, india corresponding author a b s t r a c t calcium phosphate, aluminum phosphate and ferrous phosphate and thus becomes unavailable to plants. Isolation and identification of phosphate solubilizing bacteria psb in the present study, the collected soil samples were evaluated in vitro for p solubilising bacteria in pikovskayas pkv plates supplemented with 1. Lecturer5 department of microbiology malanakara catholic college mariagiri, kaliakkavilai 629053. Occurrence, mechanisms and their role in crop production ahmad ali khan 1, ghulam jilani 1, mohammad saleem akhtar 1, syed muhammad saqlan naqvi 2, mohammad rasheed 3 abstract plants acquire phosphorus from soil solution as phosphate anion. Jul 26, 2017 phosphate solubilizing bacteria psb can promote the dissolution of insoluble phosphorus p in soil, enhancing the availability of soluble p. The mixture was shaken in a rotary shaker for 20 min at room temperature. Organic phosphate solubilization is also called mineralization of organic phosphorus, and it occurs in soil at the expense of plant and animal remains, which contain a large amount of organic phosphorus compounds. Isolation of potential phosphate solubilizing bacteria. How to isolate phosphate solubilizing bacteria youtube. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan. The objective of the study was to explore the capabilities of psb and evaluate their efficiency to enhance growth of sugarcane plants under greenhouse condition. Occurrence and genetic diversity of phosphatesolubilizing. A total of ten bacteria isolates were tested for k.
Isolation and identification of potential phosphate solubilizing bacteria from the rhizosphere of lupinus hirsutus l. Isolation and classification of nitrogen fixing and phosphate. In this study, highthroughput isolation hti was used to identify n fixing andor p solubilizing bacteria. Guru nanak institute of research and development, guru nanak khalsa college, nathalal parekh marg, matunga e, mumbai, maharashtra, india. A study on isolation of phosphate solubilizing bacterial psb strain and fungi from salinity affected soil and production of biofertilizers n. A biologically pure phosphate solubilizing plant growth promoting rhizobacteria pgpr xsb375 was isolated from canola rhizosphere and identified as bacillus firmus is provided herein. Phosphate solubilizing bacteria psb can convert insoluble forms of phosphorus p to accessible forms. Phosphate solubilization potential of rhizosphere fungi. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants. Locally available cow dung has been used to isolate two bacterial strains, zs20 and e57.
These bacterial strains were identified by sequence analysis of 16s rrna genes as bacterial species of bacillus megaterium b. To check the ability for phosphate solubilization, production of indole acetic acid, antagonistic activity against fungal pathogen and intrinsic antibiotic resistance phosphorous solubilizing bacterial isolates were. Deshmukh2 1department of microbiology sant gadge baba amravati university, amravati india. The inoculation of p solubilizing microorganisms is a promising technique because it can increase p availability in soils fertilized with rock phosphates. Phosphorus is a major essential macronutrient for plant growth, and most of the phosphorus in soil remains in insoluble form. Plants acquire phosphorus from soil solution as phosphate anion. The selected strain was identified as aspergillus niger based on morphological and microscopic studies. Isolation of phosphate solubilizing bacteria psb from maize rhizospheric soils.
There was a correlation between final ph value and titratable acidity r. Wo2014082167a1 phosphate solubilizing rhizobacteria. Hence, previous studies have investigated phosphate solubilizing rhizobia psr as biofertilizers, in order to reduce the cost of chemical fertilizers and to decrease soil degradation and pollution 11. Isolation of phosphate solubilizing bacteria and fungi from rhizospheres soil from banana plants and its effect on the growth of amaranthus cruentus l. The collected samples were carefully packed in paper bags and stored at room temperature. Rhizobia are group bacteria with high potential of phosphate solubilization 1820. Result indicates that bacillus species is the good source of insoluble phosphate to soluble phosphate in soil. From the 14 isolated bacterial isolates 5 bacterial strains were selected.
Identification and characterization of the phosphate. Experimental studies found that the strain pssg6 was effective in phosphate solubilization with phosphate solubilization efficiency index e 375 8. Isolation and characterization of phosphate solubilizing. The present invention relates to a composition useful for qualitative screening of phosphate solubilizing microorganism, and also relates to a synergistic composition for qualitative screening of phosphate solubilizing microorganisms, based upon visual observation. Isolation, characterization and identification of potassium solubilizing bacteria from rhizosphere soil of maize zea mays. The goal of this research lies in the identification of beneficial bacteria capable of fixing nitrogen and solubilizing phosphate. To circumvent the phosphate deficiency, phosphate solubilizing microorganisms play an important role in supplying p to plants. Isolation and assessment of nitrogenfixing and phosphate.
An overview krishnananda pralhad ingle1 and dipika ashokrao padole2 biotechnology centre, dr. How to isolate phosphate solubilizing bacteria sajid shaikh. Jump to content jump to main navigation jump to main navigation. Lead bioimmobilization in contaminated mine soil by. One of the premium qualities of phosphate solubilizing bacteria is to solubilize insoluble phosphorus to make it available for plant roots to be engrossed. Characterization of phosphatesolubilizing bacteria isolated. Five bacterial strains with phosphate solubilizing ability and other plant growth promoting traits. Based on morphological observations, 16s rrna sequencing, cellular fatty acid composition analysis.
Isolation and screening of phosphate solubilizing bacterium from soil article pdf available in biotechnology. Isolation and characterization of ralstonia pickettii a. The production of organic acids by phosphate solubilizing bacteria has been well documented. Five highly efficient psb strains, h22, y11, y14, y34, and s32, were screened and isolated from an alfalfa rhizosphere in heavy metalcontaminated reclamation area in shanxi province, china. In the present work we have made efforts to isolate the phosphate solubilizing microorganism form soil followed by its biochemical characterization. This was placed in polythene bags and brought to the laboratory in ice boxes. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication.
The pgpr is capable to solubilize phosphate that enhance plant available phosphorous. Isolation and characterization of phosphofungi, and. Pdf isolation of phosphate solubilizing microorganism psms. Isolation and screening of silicate bacteria from various. Phosphate solubilizing activity of some bacterial strains. Sane and mehta showed that phosphate solubilizing bacteria and fungi in coinoculation increase the mobilization of rock phosphate and plant growth. Soil samples were collected from rhizosphere of tomato plant. Here we report isolation of ralstonia pickettii a novel phosphate solubilizing bacterium from a unique source rhizosphere soil of pomegranate as well as some other relatively less explored sources from different parts of western india.
The present study was aimed at isolating and characterizing phosphate solubilizing fungi from different rhizospheres using both solid and liquid pikovskaya pvk medium. Initially, 50 isolates were isolated on the basis of zone of clearance around their colonies on pkv plates. From the rhizosphere of chinese cabbage plants grown in yangling, we isolated a strain of psb yl6 with a strong ability to dissolve p. Isolation of phosphorussolubilizing fungus from soil to. It has been reported that higher concentrations of phosphate solubilizing bacteria are commonly found in the rhizosphere as compared to bulk soil 30. The effect of four phosphate solubilizing bacteria psb was studied on growth and aloina content of aloe barbadensis in soil containing tricalcium phosphate tcp. Sep 01, 2016 the search for life in space documentary where is the centre of the universe touch your heart 470 watching live now. The rhizosphere soil was collected from all the 10 trees of one site and pooled together to form a sampling. Experimental collection and preparation of soil samples. Isolation and characterization of phosphatesolubilizing bacteria from seagrass rhizosphere soil. Phosphate solubilization all of the isolated bacterial isolates were checked for phosphate solubilization on pikoviskaya agar plates 1618.
Oct 26, 2015 a number of bacterial and fungal isolates from soil were screened for phosphorus solubilization. Studies on regulation of pqqdependent phosphate solubilization among rhizosphere dwelling bacteria plant growth promotion can be enhanced by soil and rhizospheredwelling bacteria by a several different methods. A small number of studies investigated on the occurrence of phosphate solubilizing bacteria in the marine environment and fresh water environment like lake ecosystem 3, 4. Isolation of phosphate solubilizing bacteria from maize. Soil inoculation with phosphate solubilizing bacteria. Phosphate solubilizing microorganisms refer to a group of soil microorganisms that as components of phosphorus cycle, can release it from insoluble sources by different mechanisms. Psb could convert these insoluble phosphates into available forms for plant via acidification, chelation, exchange reactions, and production of gluconic isolation and characterization of phosphate solubilizing bacteria. Some important cultural conditions were optimized, and optimal conditions for temperature, glucose. Tofazzal islama,b, abhinandan deoraa, yasuyuki hashidokoa, atiqur rahmana, toshiaki itoa, and satoshi taharaa a graduate school of agriculture, hokkaido university, kitaku, sapporo 0608589, japan b present address.
Isolation and characterization of phosphorus solubilizing. Isolation, screening and characterization of phosphate. In search of efficient salttolerant phosphate solubilizing bacteria psb, we conducted a survey of psb that naturally colonize the rhizosphere of peanuts in xinjiang. Isolation and characterization of phosphorus solubilizing bacteria with multiple phosphorus sources utilizing capability and their potential for lead immobilization in soil wenjie wan 1,2, yin qin 1, huiqin wu 1, wenlong zuo 1, huangmei he. Rhizospheric microorganisms can increase phosphorus availability in the soil. Isolation and identification of elite phosphate solubilizing. It is important to explore a highefficient phosphatesolubilizing approach with a kind of microorganisms. Isolation and biochemical characterization of plant growth. Thus, their application can reduce the consumption of fertilizer and aid in sustainable agricultural development.
Percentage of silicate solubilizing bacteria was checked for each soil sample and calculated as follows. Isolation of strains for isolation of phosphate solubilizing microbes, 1g rhizosphere soil was suspended in 100ml of distilled water. Usually, one gram of fertile soil contains 101 to 1010 bacteria, and their live weight may exceed 2,000 kg ha1. Factors determining rock phosphate solubilization by. Highly efficient phosphate solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. Hook and halodule pinifolia miki hartog in the vellar estuary. Dec 12, 2011 phosphatesolubilizing bacterial strains 6 nos. In this study a bacterial strain was isolated from rhzhosphere soil and screened for phosphate solubilization property.
It precipitates in soil as orthophosphate or is absorbed by fe and al oxides through legend exchange. Isolation and characterization of phosphate solubilising. Us6638730b2 composition for qualitative screening of. Isolation of phosphate solubilizing bacteria psb from rhizosphere soil. Isolation and characterization of halotolerant phosphate. The mechanism behind phosphate solubilization is explained by the ability of some soil bacteria to. Attempts were made to isolate potasium solubilizing bacteria ksb from rhizosphere soil of different crops in nadia district of west bengal. The aim of the current study was to identify efficient phosphofungi that could be developed as commercial microbial agents.
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